anti mettl4  (Proteintech)

Journal: Nutrients

Article Title: Roseburia intestinalis Supplementation Could Reverse the Learning and Memory Impairment and m6A Methylation Modification Decrease Caused by 27-Hydroxycholesterol in Mice

doi: 10.3390/nu16091288

Figure Lengend Snippet: Primers used in this study.

Article Snippet: The antibodies used were as below: β-actin (ABclonal, AC026, 1:50,000, Wuhan, China), METTL4 (bioss, bs-18851R, 1:1000, Beijing, China), PSD-95 (Abcam, ab18258, 1:1000, Cambridge, UK), occludin (Abcam, ab216327, 1:1000, Cambridge, UK), claudin-1 (Abcam, ab180158, 1:1000, Cambridge, UK).

Techniques: Sequencing

Journal: Nutrients

Article Title: Roseburia intestinalis Supplementation Could Reverse the Learning and Memory Impairment and m6A Methylation Modification Decrease Caused by 27-Hydroxycholesterol in Mice

doi: 10.3390/nu16091288

Figure Lengend Snippet: Levels of the m6A modification and its related enzyme expression. ( A ) m6A methylation in the brain cortex (ng/200ng RNA). ( B ) Western blot result of METTL4. ( C ) METTL4 mRNA relative expression level in brain cortex. ( D ) METTL4 protein expression level in the brain cortex. ( E ) METTL14 mRNA relative expression level in the brain cortex, ( F ) YTHDF-1 mRNA relative expression level in the brain cortex. ( G ) FTO mRNA relative expression level in the brain cortex. ( H ) WTAP mRNA relative expression level in the brain cortex. n = 4–5 mice/group. All the data are presented as means ± SEM. * p < 0.05. ** p < 0.01. *** p < 0.001. ns p > 0.05.

Article Snippet: The antibodies used were as below: β-actin (ABclonal, AC026, 1:50,000, Wuhan, China), METTL4 (bioss, bs-18851R, 1:1000, Beijing, China), PSD-95 (Abcam, ab18258, 1:1000, Cambridge, UK), occludin (Abcam, ab216327, 1:1000, Cambridge, UK), claudin-1 (Abcam, ab180158, 1:1000, Cambridge, UK).

Techniques: Modification, Expressing, Methylation, Western Blot

Journal: Scientific Reports

Article Title: METTL3/IGF2BP2 axis affects the progression of colorectal cancer by regulating m6A modification of STAG3

doi: 10.1038/s41598-023-44379-x

Figure Lengend Snippet: Antibodies used in the study are shown.

Article Snippet: METTL14 , 1: 1000 , Rabbit , 55 , orb28269, Biorbyt.

Techniques:

Journal: Scientific Reports

Article Title: METTL3/IGF2BP2 axis affects the progression of colorectal cancer by regulating m6A modification of STAG3

doi: 10.1038/s41598-023-44379-x

Figure Lengend Snippet: METTL3 mediated m6A methylation of STAG3. ( A ) In CRC and adjacent normal tissues, the protein expression levels of METTL3, METTL14, ALKBH5, and FTO were determined by WB. ( B,C ) The mRNA and protein expression levels of METTL3 in HCT116 cells were measured using qRT-PCR and WB. ( D ) The STAG3 m6A modification level in HCT116 cells was evaluated by Me-RIP. *P < 0.05 vs. normal, & P < 0.05 vs. sh-NC, # P < 0.05 vs. oe-NC. Superscript a: P < 0.05, superscript b: P < 0.01, superscript d: P < 0.0001.

Article Snippet: METTL14 , 1: 1000 , Rabbit , 55 , orb28269, Biorbyt.

Techniques: Methylation, Expressing, Quantitative RT-PCR, Modification